1P5G
Enzyme-ligand complex of P. aeruginosa PMM/PGM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.591, 74.372, 85.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 * |
| R-factor | 0.16177 |
| Rwork | 0.160 |
| R-free | 0.19000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k35 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.477 |
| Number of reflections | 59418 | |
| <I/σ(I)> | 41.8 | 5.6 |
| Completeness [%] | 98.7 | 87.7 |
| Redundancy | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | Regni, C.A., (2000) Acta Crystallogr, D56, 761. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-15 (mg/ml) | |
| 2 | 1 | drop | MOPS | 10 (mM) | pH7.0 |
| 3 | 1 | reservoir | sodium potassium tartrate | 1.4 (M) | |
| 4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
