1P56
Duplication-extension of Helix A of T4 lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.300, 60.300, 96.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.214 |
| Rwork | 0.208 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lzm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 13.607 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 1.900 |
| High resolution limit [Å] | 1.700 | 1.790 |
| Number of reflections | 22444 | |
| <I/σ(I)> | 7.2 | 5.1 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 35% PEG 4000, 50mM phosphate buffer, 5% isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
