1P4N
Crystal Structure of Weissella viridescens FemX:UDP-MurNAc-pentapeptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979711 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.201, 100.610, 46.538 |
| Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
| Resolution | 15.570 - 1.900 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.20700 |
| Structure solution method | difference fourier synthesis |
| Starting model (for MR) | 1ne9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.570 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 * | 0.190 * |
| Total number of observations | 102033 * | |
| Number of reflections | 27221 | |
| <I/σ(I)> | 7.5 | 3.7 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 298 | PEG 2K, NaCl, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 25 (%) | |
| 3 | 1 | reservoir | 300 (mM) | ||
| 4 | 1 | reservoir | cacodylate | 100 (mM) | pH6.5 |
