1P48
REVERSE PROTONATION IS THE KEY TO GENERAL ACID-BASE CATALYSIS IN ENOLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-10-23 |
| Detector | BRUKER PROTEUM R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.800, 114.500, 73.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | LSCALE ((Bruker Nonius)) |
| Data scaling software | SAINT |
| Phasing software | MOLREP ((CCP4)) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 * | 0.189 * |
| Total number of observations | 231529 * | |
| Number of reflections | 61265 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 293 | PEG 8000, potassium chloride, HEPPS, pH 8.0, Batch, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | PEG8000 | 13 (%) | |
| 3 | 1 | 1 | 0.25 (M) | ||
| 4 | 1 | 1 | HEPPS/KCH | 25 (mM) | pH8.0 |
| 5 | 1 | 1 | PEP | 3.5 (mM) | |
| 6 | 1 | 1 | 3.5 (mM) |
