1P37

T4 LYSOZYME CORE REPACKING BACK-REVERTANT L102M/CORE10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.490, 59.490, 96.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.700 - 1.570
R-factor	0.182
Rwork	0.180
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.018 *
RMSD bond angle	2.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.700	1.650
High resolution limit [Å]	1.570	1.570
Rmerge	0.061	0.029
Number of reflections	25157
<I/σ(I)>	6.7	2.9
Completeness [%]	90.6	75 *
Redundancy	3.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol