1P32

CRYSTAL STRUCTURE OF HUMAN P32, A DOUGHNUT-SHAPED ACIDIC MITOCHONDRIAL MATRIX PROTEIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	95
Detector technology	CCD
Collection date	1998-01
Detector	BRANDEIS
Wavelength(s)	0.95,0.9786,0.9789,1.0
Spacegroup name	P 1 21 1
Unit cell lengths	58.625, 56.481, 93.831
Unit cell angles	90.00, 95.99, 90.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.173 *
Rwork	0.173
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	21.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.045	0.097
Total number of observations	170171 *
Number of reflections	27284
<I/σ(I)>	15.4	4.5
Completeness [%]	92.6	59.8
Redundancy	6.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	16 *	pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	glycerol	10 (%)
3	1	reservoir	MPD	25 (%)
4	1	reservoir	sodium acetate	5-10 (mM)