1P32
CRYSTAL STRUCTURE OF HUMAN P32, A DOUGHNUT-SHAPED ACIDIC MITOCHONDRIAL MATRIX PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1998-01 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.95,0.9786,0.9789,1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.625, 56.481, 93.831 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.173 * |
| Rwork | 0.173 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.045 | 0.097 |
| Total number of observations | 170171 * | |
| Number of reflections | 27284 | |
| <I/σ(I)> | 15.4 | 4.5 |
| Completeness [%] | 92.6 | 59.8 |
| Redundancy | 6.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 16 * | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | reservoir | glycerol | 10 (%) | |
| 3 | 1 | reservoir | MPD | 25 (%) | |
| 4 | 1 | reservoir | sodium acetate | 5-10 (mM) |
