1P2T
H-Ras 166 in Aqueous mother liqour, RT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.170, 40.170, 161.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.210 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 121p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.210 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 10879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | 298 | Scherer, A., (1989) J.Mol.Biol., 206, 257. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3-4 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 64 (mM) | |
| 3 | 1 | 1 | dithioerithritol | 1 (mM) | |
| 4 | 1 | 1 | 10 (mM) | ||
| 5 | 1 | 1 | sodium azide | 1 (mM) | |
| 6 | 1 | 1 | PEG1450 | 20 (%(w/v)) |
