1P2S

H-Ras 166 in 50% 2,2,2 triflouroethanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	273
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	40.330, 40.330, 161.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.560 - 2.400 *
R-factor	0.188
Rwork	0.188
R-free	0.22500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	121p
RMSD bond length	0.007
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.290
High resolution limit [Å]	2.400 *
Number of reflections	8311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	298	Scherer, A., (1989) J.Mol.Biol., 206, 257. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3-4 (mg/ml)
2	1	1	Tris-HCl	64 (mM)
3	1	1	dithioerithritol	1 (mM)
4	1	1		10 (mM)
5	1	1	sodium azide	1 (mM)
6	1	1	PEG1450	20 (%(w/v))