1P27

Crystal Structure of the Human Y14/Magoh complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.7114, 1.7113, 1.6755
Spacegroup name	P 1 21 1
Unit cell lengths	47.150, 108.455, 50.942
Unit cell angles	90.00, 90.24, 90.00

Refinement procedure

Resolution	54.000 * - 2.000
R-factor	0.22232
Rwork	0.220
R-free	0.26900 *
Structure solution method	MAD
RMSD bond length	0.017 *
RMSD bond angle	1.650 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000 *
High resolution limit [Å]	2.000
Rmerge	0.070 *	0.225 *
Number of reflections	33810
Completeness [%]	97.0	83 *
Redundancy	6.5	4.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	sodium cacodylate	100 (mM)	pH6.2
3	1	reservoir	MPD	25 (%)
4	1	reservoir		20 (mM)