1P1N

GluR2 Ligand Binding Core (S1S2J) Mutant L650T in Complex with Kainate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	62.386, 88.741, 48.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.610 - 1.600
R-factor	0.268
Rwork	0.235
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.410 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.063	0.110
Number of reflections	33935
<I/σ(I)>	20.6
Completeness [%]	94.2	68.3
Redundancy	4.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	4 *	PEG 4000, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	kainate	20 (mM)