1P0K
IPP:DMAPP isomerase type II apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 115.833, 115.833, 141.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.22100 * |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.470 |
| Number of reflections | 72016 | |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEG 400, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 0.02 (%(w/v)) | pH8.0 | |
| 4 | 1 | reservoir | tricine | 70 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 30 (mM) | |
| 6 | 1 | reservoir | 170 (mM) | ||
| 7 | 1 | reservoir | PEG400 | 14 (%(v/v)) | |
| 8 | 1 | reservoir | PEG8000 | 2.5 (%(w/v)) | pH8.7 |
