1P03

STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	66.350, 66.350, 80.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.150
R-factor	0.142
RMSD bond length	0.140 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.150
Completeness [%]	89.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *		seeding, refer to Bone, R. et al (1987). Biochemistry, 27, 7609-7614. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		1.3 (M)
2	1	drop	Tris-sulphate	20 (mM)