1OZV
Crystal structure of the SET domain of LSMT bound to Lysine and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 131.960, 156.900, 267.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.650 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mlv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.056 * | 0.494 * |
| Number of reflections | 79741 | 7902 * |
| <I/σ(I)> | 25.9 | 2.51 |
| Completeness [%] | 98.9 | 99 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 25 * | 0.95-1.10 M Sodium Acetate, 100 mM Lysine Acetate, 1 mM TCEP, 400 uM S-adenosylhomocysteine, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.95-1.10 (M) | |
| 3 | 1 | reservoir | AdoHcy | 4000 (nM) | |
| 4 | 1 | reservoir | TCEP | 1 (mM) | |
| 5 | 1 | reservoir | Lys | 100 (mM) | or MeLys, pH6.8 |
