1OZJ
Crystal structure of Smad3-MH1 bound to DNA at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.600, 60.400, 71.600 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| Rwork | 0.210 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.265 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 * | 0.166 * |
| Total number of observations | 68907 * | |
| Number of reflections | 14360 | |
| Completeness [%] | 95.9 | 82.5 |
| Redundancy | 4.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Shi, Y., (1998) Cell, 94, 585. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | citrate | ||
| 2 | 1 | 1 | PEG200 | ||
| 3 | 1 | 1 | ammonium acetate | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | magnesium chloride | ||
| 6 | 1 | 2 | ammonium acetate | ||
| 7 | 1 | 2 | magnesium chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | reservoir | PEG2000 | 28 (%) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 6 | 1 | drop | protein | 1 (mM) |
