1OYS

Crystal Structure of the Phosphorolytic Exoribonuclease RNase PH from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 41 21 2
Unit cell lengths	63.230, 63.230, 203.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.258
R-free	0.28400 *
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.047 *	0.206 *
Number of reflections	16768	817 *
Completeness [%]	98.8 *	100 *
Redundancy	4.4 *	5.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.2 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15.3 (mg/ml)
2	1	reservoir	sodium citrate	1 (M)	pH6.2
3	1	reservoir	PEG400	10 (%)