1OYJ
Crystal structure solution of Rice GST1 (OsGSTU1) in complex with glutathione.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.829, 91.129, 165.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.22675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.111 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.001 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.102 | |
| Number of reflections | 78138 | |
| Completeness [%] | 91.7 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.74 | 293 | Thom, R., (2002) Biochemistry, 41, 7008. * |
