1OYE
Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Spacegroup name | H 3 2 |
Unit cell lengths | 145.109, 145.109, 517.161 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.600 - 3.500 * |
R-factor | 0.26 |
Rwork | 0.257 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.050 | |
High resolution limit [Å] | 3.500 * | 3.500 * |
Rmerge | 0.087 * | 0.546 * |
Total number of observations | 596530 * | |
Number of reflections | 49289 * | |
Completeness [%] | 100.0 * | 100 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.6 * | 25 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 35 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
3 | 1 | drop | DDM | 0.1 (%) | |
4 | 1 | drop | dithiothreitol | 20 (mM) | |
5 | 1 | reservoir | PEG4000 | 7 (%) | |
6 | 1 | reservoir | sodium citrate | 20-40 (mM) | pH5.6 or 6.5 |
7 | 1 | reservoir | 20-50 (mM) | ||
8 | 1 | reservoir | glycerol | 10 (%) |