1OYA

OLD YELLOW ENZYME AT 2 ANGSTROMS RESOLUTION: OVERALL STRUCTURE, LIGAND BINDING AND COMPARISON WITH RELATED FLAVOPROTEINS

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	142.880, 142.880, 43.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.18
Rwork	0.180
R-free	0.24100
RMSD bond length	0.030 *
RMSD bond angle	2.400 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.040 *
Number of reflections	28528
Completeness [%]	91.1
Redundancy	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *	Fox, K.M., (1993) J. Mol. Biol., 234, 502. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		10 (mM)
4	1	drop	phrnylmethylsulfonyl fluoride	0.01 (M)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir	PEG400	35 (%(w/v))
7	1	reservoir		0.2 (M)