1OY9
Structural Basis of Multiple Drug Binding Capacity of the AcrB Multidrug Efflux Pump
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.676, 144.676, 517.512 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.600 - 3.800 |
| R-factor | 0.286 |
| Rwork | 0.283 |
| R-free | 0.34400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.580 | |
| High resolution limit [Å] | 3.800 * | 3.800 * |
| Rmerge | 0.101 * | 0.442 * |
| Total number of observations | 280672 * | |
| Number of reflections | 36493 * | |
| Completeness [%] | 99.0 * | 100 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 * | 25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 3 | 1 | drop | DDM | 0.1 (%) | |
| 4 | 1 | drop | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 7 (%) | |
| 6 | 1 | reservoir | sodium citrate | 20-40 (mM) | pH5.6 or 6.5 |
| 7 | 1 | reservoir | 20-50 (mM) | ||
| 8 | 1 | reservoir | glycerol | 10 (%) |
