1OXZ
Crystal Structure of the Human GGA1 GAT domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 83.400, 83.400, 68.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 2.800 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.28500 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 19.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.044 | 0.489 |
Total number of observations | 56805 * | |
Number of reflections | 6561 * | 676 * |
<I/σ(I)> | 42.1 | 4.2 |
Completeness [%] | 96.1 * | 100 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | lithium sulfate, MES, ethylene glycol , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 250 (mM) | ||
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | beta-mercaptoethanol | 0.1 (%(v/v)) | |
4 | 1 | drop | protein | 5 (mg/ml) | |
5 | 1 | reservoir | 0.4 (M) | ||
6 | 1 | reservoir | MES | 0.1 (M) | pH5.5 |
7 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
8 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%(v/v)) |