1OXT
Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.032, 146.641, 178.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 * - 2.100 |
| R-factor | 0.21742 |
| Rwork | 0.214 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 * | 0.336 * |
| Number of reflections | 69924 | |
| <I/σ(I)> | 20.5 | 3 |
| Completeness [%] | 95.4 | 71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 291 | PEG 3350, PEG 400, Tris, NaCl, glycerol, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.25 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 15 (%) | |
| 2 | 1 | drop | MES | 20 (mM) | pH6.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
| 7 | 1 | reservoir | PEG400 | 15 (%(w/v)) | |
| 8 | 1 | reservoir | Tris | 0.1 (M) | pH8.3 |
| 9 | 1 | reservoir | 0.5 (M) |
