1OXQ
Structure and Function Analysis of Peptide Antagonists of Melanoma Inhibitor of Apoptosis (ML-IAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2686 |
| Spacegroup name | P 32 |
| Unit cell lengths | 83.189, 83.189, 93.612 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.16345 |
| Rwork | 0.161 |
| R-free | 0.21800 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1oxn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.379 |
| Number of reflections | 62709 | |
| <I/σ(I)> | 21.8 | 3.4 |
| Completeness [%] | 97.2 | 100 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 298 | sodium acetate, PEG 300, DTT, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 10 (mM) | pH6.5 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.0 |
| 4 | 1 | reservoir | PEG300 | 5 (%(v/v)) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
