1OXM
STRUCTURE OF CUTINASE
Experimental procedure
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.200, 69.200, 73.300 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE AT 1.6 ANGSTROMS (PDB ENTRY 1CUS) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.325 |
| Data reduction software | MARSCALE |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.177 |
| Total number of observations | 45670 * | |
| Number of reflections | 15569 | |
| <I/σ(I)> | 25.13 | 6.9 |
| Completeness [%] | 93.8 | 67.7 |
| Redundancy | 2.94 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | Abergel, C., (1990) J. Mol. Biol., 215, 215. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cutinase | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | |
| 3 | 1 | drop | PEG6000 | 14 (%(w/v)) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | PEG6000 | 14 (%(w/v)) |
