1OXF

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-11
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 21 21 21
Unit cell lengths	51.591, 62.824, 65.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 1.750 *
R-factor	0.209
Rwork	0.209
R-free	0.26100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.562 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	1.740
High resolution limit [Å]	1.750 *	1.750 *
Rmerge	0.038 *	0.224 *
Number of reflections	24503
Completeness [%]	89.9 *	88.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG 1000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 25K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ECFP	19 (mg/ml)
3	1	drop	PEG1000	14 (%(w/v))
4	1	reservoir	HEPES	0.1 (M)	pH7.5
5	1	reservoir	PEG8000	5 (%)
6	1	reservoir	ethylene gylcol	10 (%)