1OXD
Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.952, 62.768, 69.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 1.150 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.22800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.542 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | 1.220 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.039 * | 0.247 * |
| Number of reflections | 73514 | |
| Completeness [%] | 92.4 | 84 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 1000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ECFP | 19 (mg/ml) | |
| 3 | 1 | drop | PEG1000 | 14 (%(w/v)) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 5 | 1 | reservoir | PEG8000 | 5 (%) | |
| 6 | 1 | reservoir | ethylene gylcol | 10 (%) |
