1OX1
crystal structure of the bovine trypsin complex with a synthetic 11 peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.700, 63.140, 69.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.310 - 2.000 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sbw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 18579 | |
| <I/σ(I)> | 9.1 | 3.3 |
| Completeness [%] | 95.6 | 89 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | sodium sulfate, sodium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
