1OWB

Three Dimensional Structure Analysis Of The Variant R109L NADH Complex of Type II Citrate Synthase From E. Coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-07-10
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	165.001, 165.001, 157.774
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
Rwork	0.162
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1K3P
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Rmerge	0.083
Number of reflections	76417
Completeness [%]	92.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP