1OW1

Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	41.300, 61.980, 68.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.214
Rwork	0.214
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.068 *	0.082 *
Total number of observations	258694 *
Number of reflections	16853
<I/σ(I)>	17.1
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	PEG 4000, glycerol, Tris-HCl, NaCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)	pH7.5
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	glycerol	5 (%)