1OVZ
Crystal structure of human FcaRI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 41 |
| Unit cell lengths | 158.150, 158.150, 39.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 3.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.29100 * |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.077 | 0.404 |
| Total number of observations | 154491 * | |
| Number of reflections | 10246 * | |
| <I/σ(I)> | 22.4 | 4.9 |
| Completeness [%] | 99.7 * | 100 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | PEG 8000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 3 | 1 | drop | PEG6000 | 20 (%) | |
| 4 | 1 | drop | sodium phosphate | 0.1 (M) | pH6.0 |
