1OVW

ENDOGLUCANASE I COMPLEXED WITH NON-HYDROLYSABLE SUBSTRATE ANALOGUE

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1995-08-29
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	68.155, 78.279, 142.461
Unit cell angles	90.00, 96.89, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.197 *
Rwork	0.197
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE (2 MOLECULES IN ASYMMETRIC UNIT)
RMSD bond length	0.012
RMSD bond angle	0.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.840
High resolution limit [Å]	2.700	2.700
Rmerge	0.118	0.369
Total number of observations	82217 *
Number of reflections	38998
<I/σ(I)>	7.4	2.49
Completeness [%]	94.7	94.96
Redundancy	2.1	1.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		22 % PEG 8K, 0.2 M MAGNESIUM CHLORIDE, PH 6.5 FOR 0.1 M MOPS METHOD: HANGING DROP VAPOUR DIFFUSION THE NATIVE CRYSTALS WERE SOAKED FOR 1 HOUR IN STABILISING SOLUTION CONTAINING 5 MM OF THIO-CELLOPENTAOSE, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	14 (mg/ml)
2	1	drop	MOPS	100 (mM)
3	1	reservoir	PEG8000	22 (%)
4	1	reservoir		200 (mM)