1OVG

M64V PNP +MePdr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	170
Detector technology	CCD
Collection date	2001-12-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.948
Spacegroup name	P 61 2 2
Unit cell lengths	120.800, 120.800, 240.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 2.200
R-factor	0.229
Rwork	0.229
R-free	0.25600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.250 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.087	0.287
Number of reflections	52262 *
<I/σ(I)>	6.5	2.2
Completeness [%]	98.8 *	98.7 *
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	295	50mM sodium citrate, 30% ammonium sulfate , pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (mM)	pH8.0
2	1	drop	protein	35 (mg/ml)
3	1	reservoir	ammonium sulfate	30 (%)
4	1	reservoir	citrate	50 (mM)	pH5.4