1OV3

Structure of the p22phox-p47phox complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	132.660, 57.810, 45.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.232
Rwork	0.229
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ng2
RMSD bond length	0.010 *
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.370
Number of reflections	225037
<I/σ(I)>		3.6
Completeness [%]	99.2	99.1
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	18 *	1 M Na-Citrate, 0.1 M Na-cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-35 (mg/ml)
2	1	drop	HEPES	20 (mM)	pH7.0
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG3000	12 (%)
6	1	reservoir	sodium citrate		pH5.0