1OUZ
Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.799, 60.107, 181.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.410 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.27100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ihf |
| RMSD bond length | 0.003 * |
| RMSD bond angle | 0.690 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 13356 |
| Completeness [%] | 85.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 19 * | PEG5000-MME, glycerol, Tris-HCl, magnesium chloride, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG5000-MME | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | magnesium chloride | ||
| 5 | 1 | 1 | sodium chloride | ||
| 6 | 1 | 2 | PEG5000-MME | ||
| 7 | 1 | 2 | magnesium chloride | ||
| 8 | 1 | 2 | sodium chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.7 (mg/ml) | |
| 10 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | glycerol | 8 (%) | |
| 6 | 1 | reservoir | glycerol | 15 (%) | |
| 7 | 1 | reservoir | PEG5000 MME | 25 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.5 |
| 9 | 1 | reservoir | 10 (mM) |
