1OUZ

Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	46.799, 60.107, 181.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.410
R-factor	0.2426
Rwork	0.241
R-free	0.27100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ihf
RMSD bond length	0.003 *
RMSD bond angle	0.690 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.400
Number of reflections	13356
Completeness [%]	85.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	19 *	PEG5000-MME, glycerol, Tris-HCl, magnesium chloride, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG5000-MME
2	1	1	glycerol
3	1	1	Tris-HCl
4	1	1	magnesium chloride
5	1	1	sodium chloride
6	1	2	PEG5000-MME
7	1	2	magnesium chloride
8	1	2	sodium chloride

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.7 (mg/ml)
10	1	reservoir		50 (mM)
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	drop		0.1 (M)
4	1	drop	EDTA	0.1 (mM)
5	1	drop	glycerol	8 (%)
6	1	reservoir	glycerol	15 (%)
7	1	reservoir	PEG5000 MME	25 (%)
8	1	reservoir	Tris-HCl	50 (mM)	pH7.5
9	1	reservoir		10 (mM)