1OUW
Crystal structure of Calystegia sepium agglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.562, 51.792, 79.780 |
| Unit cell angles | 104.99, 94.36, 94.85 |
Refinement procedure
| Resolution | 25.000 - 1.370 |
| R-factor | 0.1531 |
| Rwork | 0.153 |
| R-free | 0.18200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 1.370 | |
| Rmerge | 0.022 * | 0.168 * |
| Total number of observations | 370064 * | |
| Number of reflections | 92547 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 94.0 | 91.2 * |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 20 * | 20-30% PEG 4K, 0.2 imidazole/malate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 20-30 (%) | |
| 5 | 1 | reservoir | imidazole/malate | 0.2 (M) | pH6.0 |
