1OUQ
Crystal structure of wild-type Cre recombinase-loxP synapse
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.500, 165.600, 193.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nzb |
| RMSD bond length | 0.022 |
| RMSD bond angle | 21.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 51910 | |
| Completeness [%] | 94.7 | 86.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 20 * | NaCl, EDTA, Glycerol, HEPES, MgCl2, PEG 2000, MME, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MME | ||
| 10 | 1 | 2 | EDTA | ||
| 11 | 1 | 2 | Glycerol | ||
| 12 | 1 | 2 | PEG 2000 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | EDTA | ||
| 6 | 1 | 1 | Glycerol | ||
| 7 | 1 | 1 | PEG 2000 | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Cre | 0.13 (mM) | |
| 2 | 1 | drop | annealed oligo | 0.15 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%(w/v)) | |
| 6 | 1 | drop | HEPES-NaOH | 50 (mM) | pH7.6 |
| 7 | 1 | drop | 75 (mM) | ||
| 8 | 1 | drop | PEG2000 MME | 12-15 (%(w/v)) |
