1OUO

Crystal structure of the periplasmic endonuclease Vvn

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9796, 0.9600
Spacegroup name	P 1 21 1
Unit cell lengths	49.115, 40.099, 50.631
Unit cell angles	90.00, 90.53, 90.00

Refinement procedure

Resolution	40.000 * - 2.300
Rwork	0.186
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.075 *	0.146 *
Total number of observations	62270 *
Number of reflections	9127
<I/σ(I)>	24.38	9.57
Completeness [%]	96.0	96
Redundancy	6.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG8000, ammonium acetate, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	30 (%)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	magnesium acetate	0.01 (M)
4	1	reservoir	sodium cacodylate	0.05 (M)	pH6.5