1OUO
Crystal structure of the periplasmic endonuclease Vvn
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9796, 0.9600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.115, 40.099, 50.631 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.300 |
| Rwork | 0.186 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.280 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 * | 0.146 * |
| Total number of observations | 62270 * | |
| Number of reflections | 9127 | |
| <I/σ(I)> | 24.38 | 9.57 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 6.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, ammonium acetate, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 30 (%) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | magnesium acetate | 0.01 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.05 (M) | pH6.5 |
