1OUN

CRYSTAL STRUCTURE OF NUCLEAR TRANSPORT FACTOR 2 (NTF2)

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.000, 58.100, 88.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.300
R-factor	0.158 *
Rwork	0.158
R-free	0.23000
Structure solution method	SINGLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	SHELX
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.059	0.175
Total number of observations	90240 *
Number of reflections	12825
<I/σ(I)>	9	4.1
Completeness [%]	96.9	94.7
Redundancy	5.4 *	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%(w/v))
2	1	reservoir	magnesium acetate	25-100 (mM)
3	1	reservoir	sodium acetate	100 (mM)