1OUL

Structure of the AAA+ protease delivery protein SspB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	113
Detector technology	CCD
Collection date	2002-06-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97898, 0.97939, 0.96866
Spacegroup name	P 31 2 1
Unit cell lengths	78.883, 78.883, 105.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.720 - 2.200
R-factor	0.256
Rwork	0.256
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	25.000 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	22666
Completeness [%]	99.9	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6 *	20 *	sodium formate, sodium cacodylate, ammonium sulfate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.7 (mg/ml)
2	1	reservoir	calcium acetate	0.24 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH5.6
4	1	reservoir	PEG8000	12 (%(w/v))