1OU8
structure of an AAA+ protease delivery protein in complex with a peptide degradation tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-09-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92108 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.020, 69.020, 104.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.980 - 1.600 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ou9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.107 * | 0.634 * |
| Total number of observations | 418776 * | |
| Number of reflections | 38849 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 * | 20 * | sodium citrate, sodium cacodylate, sodium thiocyanate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.7 (mg/ml) | |
| 2 | 1 | reservoir | calcium acetate | 0.24 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH5.6 |
| 4 | 1 | reservoir | PEG8000 | 12 (%(w/v)) |
