1OU0

precorrin-8X methylmutase related protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2002-02-07
Detector	SBC-2
Wavelength(s)	0.97891, 0.97878, 0.99187
Spacegroup name	P 21 21 21
Unit cell lengths	72.450, 86.820, 120.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.400 * - 2.100
R-factor	0.2027
Rwork	0.202
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	d*TREK
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.240
High resolution limit [Å]	2.100
Rmerge	NaN *
Number of reflections	43633
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.6 *	298	PEG3350, di-NH4 tartrate, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	14 (%)
2	1	reservoir	di-ammonium tartrate	0.5 (M)
3	1	reservoir	glycerol	4 (%)	pH6.6