1OTT
Structure of the Escherichia coli ClC Chloride channel E148A mutant and Fab Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.614, 95.719, 169.819 |
| Unit cell angles | 90.00, 131.45, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 3.000 |
| R-factor | 0.296 |
| Rwork | 0.296 |
| R-free | 0.33800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SDMS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.492 |
| Number of reflections | 52212 | |
| <I/σ(I)> | 14.8 | 2.1 |
| Completeness [%] | 93.7 | 76.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 20 * | PEG 300, sodium chloride, glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG300 | 28-31 (%) | |
| 2 | 1 | reservoir | glycine | 50 (mM) | pH9.5 |
| 3 | 1 | reservoir | 100 (mM) |
