1OTS
Structure of the Escherichia coli ClC Chloride channel and Fab Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 229.936, 93.870, 168.536 |
Unit cell angles | 90.00, 131.72, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.500* |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FAB fragment 1K4C |
RMSD bond length | 0.010 * |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.048 | 0.569 |
Number of reflections | 88082 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 96.6 | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 20 * | PEG 300, sodium chloride, glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG300 | 28-31 (%) | |
2 | 1 | reservoir | glycine | 50 (mM) | pH9.5 |
3 | 1 | reservoir | 100 (mM) |