1OTI
E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P65 AT 295K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 |
| Unit cell lengths | 40.987, 40.987, 117.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.133 |
| Rwork | 0.131 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.550 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.088 | 0.568 |
| Number of reflections | 21402 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 95.4 | 96.8 |
| Redundancy | 23.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 2000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
