1OT8
Structure of the Ankyrin Domain of the Drosophila Notch Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.629, 73.629, 341.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.17947 |
| Rwork | 0.177 |
| R-free | 0.20100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 * | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.048 * | 0.138 * |
| Number of reflections | 62729 | |
| Completeness [%] | 96.9 * | 93.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | MPD, Magnesium Acetate, Sodium Cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
| 4 | 1 | reservoir | MPD | 15-20 (%(v/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5-7.0 |
