1OT8

Structure of the Ankyrin Domain of the Drosophila Notch Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Wavelength(s)	1.1000
Spacegroup name	P 43 21 2
Unit cell lengths	73.629, 73.629, 341.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.17947
Rwork	0.177
R-free	0.20100 *
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.430 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000 *
High resolution limit [Å]	2.000
Rmerge	0.048 *	0.138 *
Number of reflections	62729
Completeness [%]	96.9 *	93.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	298	MPD, Magnesium Acetate, Sodium Cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop		150 (mM)
3	1	drop	Tris-HCl	25 (mM)	pH8.0
4	1	reservoir	MPD	15-20 (%(v/v))
5	1	reservoir	magnesium acetate	200 (mM)
6	1	reservoir	sodium cacodylate	100 (mM)	pH6.5-7.0