1OSP

CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	IMAGE PLATE
Collection date	1995-10-27
Detector	MARRESEARCH
Wavelength(s)	0.95, 1.1
Spacegroup name	P 21 21 21
Unit cell lengths	89.100, 91.700, 100.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.950
R-factor	0.229
Rwork	0.229
R-free	0.29500
RMSD bond length	0.007
RMSD bond angle	1.614
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.950 *	1.950 *
Rmerge	0.048	0.216 *
Total number of observations	254719 *
Number of reflections	58870
Completeness [%]	97.1	88.3 *
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)
2	1	reservoir	PEG3300	4-10 (%(w/v))
3	1	reservoir		80 (mM)