1OSI
STRUCTURE OF 3-ISOPROPYLMALATE DEHYDROGENASE
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.500, 86.700, 112.100 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.360 |
| Data reduction software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.054 |
| Number of reflections | 27930 |
| Completeness [%] | 93.8 |
| Redundancy | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.6 * | THE STRUCTURE SOLVED USING CRYSTALS GROWN FROM AMMONIUM SULFATE WAS DEPOSITED AS 1IPD AT PDB. THE CRYSTALLIZATION CONDITION IN THIS STRUCTURE WAS SIMILAR AS THE MUTANT A172L., pH 4.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 20 (mM) | |
| 2 | 1 | reservoir | PEG4000 | 6.5 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
