1OSE

Porcine pancreatic alpha-amylase complexed with acarbose

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	70.600, 114.700, 118.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.176
Rwork	0.176
R-free	0.22200
RMSD bond length	0.024
RMSD bond angle	24.790 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Rmerge	0.157
Total number of observations	450986 *
Number of reflections	41389
Completeness [%]	96.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *	White, J.L., (1982) FEBS Lett., 148, 87. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	EDTA	5 (mM)
4	1	drop	DTE	1-2 (mM)
5	1	drop	PEG10000	16-18 (%(w/v))
6	1	reservoir	PEG10000	32-36 (%(w/v))