1OSA

CRYSTAL STRUCTURE OF RECOMBINANT PARAMECIUM TETRAURELIA CALMODULIN AT 1.68 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	29.890, 53.420, 25.350
Unit cell angles	93.67, 96.88, 89.24

Refinement procedure

Resolution	6.000 * - 1.680
R-factor	0.194
Rwork	0.194
RMSD bond length	0.012
RMSD bond angle	23.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.680 *
Rmerge	0.041 *
Total number of observations	35939 *
Number of reflections	16255 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.7 (mg/ml)
2	1	drop	calcium chloride	2.2 (mM)
3	1	drop	sodium cacodylate	11.1 (mM)
4	1	drop	MPD	11.1 (%(w/v))
5	1	reservoir	MPD	50 (%(w/v))