1OS8

RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	50.041, 69.824, 119.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.920 - 1.500 *
R-factor	0.196
Rwork	0.196
R-free	0.22200
Structure solution method	RIGID BODY REFINEMENT
Starting model (for MR)	1sgt
RMSD bond length	0.007
RMSD bond angle	25.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.610
High resolution limit [Å]	1.500 *	1.550
Rmerge	0.041	0.182
Number of reflections	25923 *
<I/σ(I)>	20.4	5.1
Completeness [%]	84.1 *	74.1
Redundancy	2.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.5	293	Read, R.J., (1988) J.Mol.Biol., 200, 523. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir		10 (mM)
3	1	reservoir		0.1 (mM)
4	1	drop		0.5 (M)