1OS6
Cytochrome c7 (PpcA) from Geobacter sulfurreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-18 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 32.400, 32.400, 178.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.1821 |
| Rwork | 0.182 |
| R-free | 0.22700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 4.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.061 | 0.134 |
| Number of reflections | 30830 | |
| <I/σ(I)> | 57.7 | 9.2 |
| Completeness [%] | 96.0 * | 73 * |
| Redundancy | 5.3 * | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3.5 M Ammonium sulfate, 0.25% deoxycholic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3.5 M Ammonium sulfate, 0.25% deoxycholic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3.5 M Ammonium sulfate, 0.25% deoxycholic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 72 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | pH6.0 or 7.0 | |
| 3 | 1 | reservoir | ammonium sulfate | 3.5 (M) | pH6.0 |
| 4 | 1 | reservoir | deoxycholic acid | 0.25 (%) |
